Method and monoclonal antibodies for vitamin B12 determination

Details Method and monoclonal antibodies for vitamin B12 determination Method and monoclonal antibodies for vitamin B12 determination

1995-05-16

1997-03-25

Nucker, Christine M.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435 7021, 435337, 435336, 5303882, 5303892, 4241521, 4241981, C12N 512, C07K 1618, A61K 39395

Patent

active

056143940

ABSTRACT:
A method for making an monoclonal antibody specific for vitamin B12 by immunizing mice with vitamin B12-b-acid and isolating hybridomas that produce monoclonal antibodies which have an affinity constant for B12 greater than 5.times.10.sup.9 l/mol is disclosed.

REFERENCES:
Paul WE 1993 Fundamental Immunology p. 242.
Gershman M et al 1972 Arch. Biochem Biophys. 153:407-409.
Ahrerstedt JI et al 1979 Clinica Chimica Acta 95:419-423.
Lee DSC et al 1985 Clin Biochem 18:261-266.
Sigma Chemical Co. 1990 Product No. V-9505 Product Sheet.
Biomaker V-9505 Immuno-File Product Sheet.
Galfre et al. Methods in Erz. 1981 vol. 73:3-46.

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