Method and monoclonal antibodies for vitamin B12 determination

Details Method and monoclonal antibodies for vitamin B12 determination Method and monoclonal antibodies for vitamin B12 determination

1993-10-15

1995-09-19

Ceperley, Mary E.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 75, 436532, 436815, G01N 33577

Patent

active

054515084

ABSTRACT:
For the determination of vitamin B12 a sample solution is incubated with at least two receptors R.sub.1 and R.sub.2 of which R.sub.1 mediates the binding to the solid phase and R.sub.2 is labelled, whereby a receptor is used as one of the receptors R.sub.1 or R.sub.2 which contains a monoclonal antibody capable of specific binding to B12 that has an affinity constant of at least 5.times.10.sup.9 l/mol and a receptor is used as the other receptor R.sub.1 or R.sub.2 which contains B12 or an analogue thereof, the two phases are separated and the label is measured in one of the two phases.

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patent: 4950612 (1990-08-01), Khanna et al.
patent: 5104815 (1992-04-01), Garner et al.
Sigma Chemical Co., Techical Bulletin on Product No. V-9505, Monoclonal Anti-Vitamin B.sub.12 Mouse Ascites Fluid Clone No. CD-29, Aug. 19, 1993.
Journal of Experimental Medicine, vol. 188, No. 1, May 1988, pp. 77-81, New York; R. Carmel et al, "Monoclonal Antibodies to Different Sites on Human Transcobalamin II (42709)".
Clin. Biochem. 18 (1965), pp. 261-266, David S. C. Lee, et al., "Human Serum Vitamin B12 Assay Methods-A Review".
Archives of Biochem. and Biophys. 153 (1972), pp. 407-409, Howard Gershman, et al., "Production and Specificity of Antibodies to B12 Derivatives".

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