Method and micro roller bottle for in vitro exposure of cells to

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism

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435 31, 435809, 436 63, 436810, 422102, C12Q 118, C12M 100, C12C 100, B01L 300

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active

055081746

ABSTRACT:
A culture bottle for the in vitro exposure of a cell culture to a volatile chemical comprises a closed cylindrical body defining a longitudinal axis and having an opening at each end of the body, substantially concentric with the axis, wherein each opening, comprises an aseptically sealable septum, wherein each opening has a diameter less than the diameter of the body. This bottle is used for the in vitro exposure of a cell culture to a volatile chemical by coating the inner surface of the culture bottle with the cell culture, adding a culture medium for the cells to the bottle, sealing both ends of the bottle, thereby enclosing a first atmosphere within the bottle, injecting a quantity of a second atmosphere containing the volatile chemical into the bottle through one of the septum while simultaneously providing egress for a like quantity of the first atmosphere from the bottle through one of the septum, incubating the cell culture, while rotating the bottle, for a suitable time, and determining the viability of the cell culture. Cells which do not or will not attach to the surface of the culture bottle may be attached to a carrier, such as a collagen-coated mesh and the cell-attached mesh disposed within the culture bottle.

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