Method and medium for use in detecting target microbes in situ i

Details Method and medium for use in detecting target microbes in situ i Method and medium for use in detecting target microbes in situ i

1986-06-30

1990-05-15

Deck, Randall E.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

435 34, 435 39, 435 40, 4352528, 4352536, C12Q 110, C12Q 108, C12Q 106, C12Q 104

Patent

active

049257897

ABSTRACT:
The presence or absence of a predetermined target microbe in a sample is determined by adding a testing medium to the sample, or vice versa. The testing medium provides a selective growth medium for the target microbe and includes a specific nutrient which only the target microbe can metabolize. This specific nutrient is modified by attaching a sample-altering moiety thereto, thereby converting the nutrient to a nutrient-indicator. The sample-altering moiety is activated to alter the sample only if the specific nutrient is metabolized by the target microbe. The sample-altering moiety can be a material which changes the color of the sample (visible or non-visible) or changes an electrical characteristic of the sample, or alters some other detectable characteristic of the sample. The testing media does not have to be kept sterile, and the testing procedure does not have to be performed in a sterile environment.

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Dahlen et al., Applied Microbiology, vol. 26, No. 6, Dec. 1973, pp. 863-866.
Maddocks et al., Journal of Clinical Pathology, vol. 28, 1975, pp. 686-687.
Hansen et al., Journal of Clinical Microbiology, vol. 20, No. 6, Dec. 1984, pp. 1177-1179.

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