Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Reexamination Certificate
2007-06-26
2007-06-26
Saucier, Sandra E. (Department: 1651)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
Reexamination Certificate
active
10416298
ABSTRACT:
The invention relates to a method and a medium for microbiological analysis by biochemical means involving chromogenic or fluorogenic substrates that react with enzymes (esterases) specific for the target strains.One object of the invention is to improve the sensitivity, initial translucence, stability and ease of use of such detection/identification media. For this purpose the medium according to the invention is characterized in that it is in a stable, ready-to-use liquid or gel form and in that it contains a solubilizer and stabilizer selected from fatty acid sorbitan esters, bile salts and mixtures thereof, as well as a selective activator selected from alkylsulfate salts, for example the sodium salts. The medium can comprise a solvent, for example dimethyl sulfoxide.The invention further relates to the method of obtaining this medium and to the use of products of the sodium alkylsulfate type as selective activators in such media.Application: microbiological analyses.
REFERENCES:
patent: 2 697 028 (1994-04-01), None
patent: 92/17607 (1992-10-01), None
patent: WO 92/17607 (1992-10-01), None
patent: WO 94/09152 (1994-04-01), None
patent: WO 99/41409 (1999-08-01), None
Cooke et al., “A Novel Chromogenic Ester Agar Medium for Detection ofSalmonellae”, Applied and Environmental Microbiology, vol. 65, No. 2, Feb. 1999, pp. 807-812.
International Search Report PCT/FR01/03610 dated Mar. 1, 2002.
Bio Merieux
McCracken & Frank LLP
Saucier Sandra E.
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