Method and media for the somatic embryogenesis and regeneration

Details Method and media for the somatic embryogenesis and regeneration Method and media for the somatic embryogenesis and regeneration

1992-03-10

1994-08-02

Robinson, Douglas W.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

4352401, 4352404, 43524049, 43524054, C12N 500

Patent

active

053345304

ABSTRACT:
The present invention provides a method and medium for the in vitro propagation of bamboo through organogenesis of vegetative explants from mature bamboo plants and somatic embryogenesis of the resulting organogenic callus. Optimal organogenesis is obtained by culturing vegetative explants from mature bamboo plants on first stage bamboo nutrient media comprising vitamins, MS salts, and supplemented with 1-naphthalemic acid, 6-benzylaminopurine and sucrose. Explants from the organogenic calli obtained from first stage media are cultured on second stage media (nutrient media comprising MS salts, vitamins, and supplemented with 2,4-dichlorophenoxyacetic acid, 6-benzylaminopurine and sugar) to induce somatic embryogenesis.

REFERENCES:
patent: 4000951 (1991-08-01), Simpson
patent: 4548901 (1985-10-01), Christianson et al.
patent: 5134074 (1992-07-01), Gordon et al.
Huang, et al. (1988), Bot. Bull. Academia Sinica, 29:177-182, "Tissue Culture Investigations of Bamboo".
Rao, et al. (1985), Plant Cell Report, 4:191-194, "Somatic Embryogenesis and Regeneration of Plants in the Bamboo".
Yeh M.-L. and Chang, W.-C. (1987), Plant Science, 51:93-96, "Plant Regeneration Via Somatic Embryogenesis in Mature Embryo-Derived Callus . . . ".
Huang, et al. (1989), Bot. Bull. Academia Sinica, 30:49-57, "Tissue Culture Investigations of Bamboo".
Yeh. M.-L. and Chang, W.-C. (1986), Theor. Appl. Genet., 73:161-163, "Plant Regeneration Through Somatic Embyrogenesis in Callus Culture of Green . . . ".

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