Method and media for enhancing cryopreservation of cells

Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Method of storing cells in a viable state

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

435375, 435 13, 435404, 435406, 435366, A01N 102

Patent

active

060371757

ABSTRACT:
A method of enhancing the viability of cryopreserved cells is culturing Sertoli cells in media to produce preconditioned media and adding the preconditioned media to the cells to be cryopreserved. The cells are then cryopreserved. Alternatively, a method of enhancing the viability of cryopreserved cells is co-culturing Sertoli cells and cells to be cryopreserved in media and cryopreserving both.

REFERENCES:
patent: 5082670 (1992-01-01), Gage et al.
Selawry et al., "Production of a factor, or factors, suppressing IL-2 production and T cell Proliferation by sertoli cell-enriched Preparations"Transplantation 52:846-850 (1991).
Selawry et al, (1991) Transplantation. 52(5): 846-850.
Carson et al., "Synthesis and secretion of a novel binding protein for retinol by a cell line derived from Sertoli Cells" Journal of Biological Chemistry, vol. 259, No. 5, pp. 3117-3123 (1984).
Berden et al., "Severe central-nervous system toxicity associated with cyclosporin" The Lancet 26:219-220 (1985).
Bjorklund and Stenevi, "Intracerebral neural grafting: A historical perspective" Neural grafting in the mammalian CNS, Amsterdam:Elsevier pp. 3-14 (1985).
Bjorklund, "Dopaminergic transplants in experimental parkinsonism: cellular mechanisms of graft-induced functional recovery" Current Biology, 2:683-689 (1992).
Borlorgan et al., "Cyclosporine-a increases spontaneous and dopamine agonist-induced locomotor behavior in normal rats" Cell Transplantation, vol. 4, No. 1, pp. 65-73 (1995).
Borlongan et al., "Striatal dopamine-mediated motor behavior is altered following occlusion of the middle cerebral artery" Pharm. Biochem. and Behavior, vol. 52, No. 1 pp. 225-229 (1995).
Borlongan et al., "Systemic 3-nitropropionic acid: behavioral deficits and striatal damage in adult rats" Brain Research Bulletin vol. 36, No. 6, pp. 549-556 (1995).
Cameron et al., Sertoli cells maintain spermatid viability in vitro.sup.a Cell biology of the testis and epididymis, vol. 513 Annals of the New York Academy of Sciences, pp. 419-423 (1987a).
Cameron et al., "Alterations of androgen-binding protein (ABP) in sertoli/spermatid cocultures with varying . . . " Cell biology of the testis and epididymis, New York Acad. Sci., pp. 448-451 (1987b).
Cameron et al., "Successful islet/abdominal testis transplantation does not require leydig cells.sup.1 " Transplantation, vol. 50, No. 4 pp. 649-653 (1990).
Cameron and Muffly, "Hormonal regulation of spermatid binding" Journal of Cell Science, 100:623-633 (1991).
de Groen et al., "Central nervous system toxicity after liver transplantation" The New England Journal of Medicine, 14:861-866 (1987).
Freeman et al., "The USF protocol for fetal nigral transplantation in Parkinson's Disease" Experimental Neurology, 129:6-7 (1994).
Griswold, Protein secretion by sertoli cells: general considerations Russell, L.D. and M.D. Griswold, eds. The Sertoli Cell, Cache River Press, Clearwater, Fl., pp. 195-200 (1992).
Isacson et al., "Graft-induced behavioral recovery in an animal model of huntington disease" Proc. Natl. Acad. Sci. USA, vol. 83, pp. 2728-2732 (1986).
Koutouzis et al., "Intrastriatal 3-nitropropionic acid: a behavioral assessment" NeuroReport, vol. 5, No. 17 pp. 2241-2245 (1994).
Koutouzis et al., "Systemic 3-nitropropionic acid: long-term effects on locomotor behavior" Brain Resrarch, 646:242-246 (1994).
Lindvall et al., "Transplantation in parkinson's disease: two cases of adrenal medullary grafts to the putamen" Annals of Neurology vol. 22, No. 4, pp. 457-468 (1987).
Lindvall et al., "Grafts of fetal dopamine neurons survive and improve motor function in parkinson's disease" Science, 247:574-577 (1990).
Pakzaban et al., "Increased proportion of acetylcholinesterase-rich zones and improved morphological integration in host striatum of . . . ", Brain Research, 97:13-22 (1993).
Sagen et al., "Transplants of Immunologically isolated xenogeneic chromaffin cells provide a long-term source of pain-reducing . . . " Journal of Neuroscience, 13:2415-2423 (1993).
Sanberg et al., "Transplantation into the central nervous system" R. G. Landesd Co., Boca Raton, Fl., pp. 19-21 (1994).
Selawry and Cameron, "Sertoli cell-enriched fractions in successful islet cell transplantation" Cell Transplantation, vol. 2, No. 3 pp. 123-129 (1993).
Wictorin et al., "Reformation of long axon pathways in adult rat central nervous system by human forebrain neuroblasts" Nature, vol. 347, pp. 556-558 (1990).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Method and media for enhancing cryopreservation of cells does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method and media for enhancing cryopreservation of cells, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method and media for enhancing cryopreservation of cells will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-168517

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.