Method and means for perform biochemical reactions

Chemistry: analytical and immunological testing – Optical result – With claimed manipulation of container to effect reaction or...

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436180, 435287, 435296, 435299, 435300, 220507, 422 60, 422 72, 422100, G01N 930, G01N 2103, C12M 124, C12M 118

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active

052739074

ABSTRACT:
The present invention relates to a method for performing biochemical reactions and a combination of a capillary (3) and a reaction vessel (1) for use in said method. The reagent capillaries (3) contain frozen reagents (6-13) separated from each other by air or an inert fluid. At use, the reagent capillaries (3) are placed in a reaction vessel (1) to be thawed and then the contents are centrifugated to the bottom of the reaction vessel (1). The invention is intended for use in all types of biochemical standard reactions and diagnostic tests in which the reagents cannot be mixed in advance. It is particularly suitable for PCR diagnostics but is also especially beneficial when handling radioactive reagents, e.g. labeled nucleotides, for sequencing reactions, etc.

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patent: 4092113 (1978-05-01), Hardy
patent: 4104025 (1978-08-01), Retzer
patent: 4123224 (1978-10-01), Givnei et al.
patent: 4690670 (1987-09-01), Nielsen
patent: 4954264 (1990-09-01), Smith
patent: 4956298 (1990-09-01), Diekmann
patent: 5069336 (1991-12-01), Mauthe

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