Method and kits for titering adeno-associated virus vectors

Details Method and kits for titering adeno-associated virus vectors Method and kits for titering adeno-associated virus vectors

2005-01-11

2005-01-11

Leary, Louise N. (Department: 1654)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

C435S004000, C435S975000

Reexamination Certificate

active

06841357

ABSTRACT:
A method of titering adeno-associated virus particles in a sample, said method comprising the steps of contacting target cells with a DNA synthesis inhibitor and an agent that increases the activity of the CMV immediate early promoter; contacting target cells treated as in step (a) with a sample containing adeno-associated virus particles; and determining the number of target cells infected by said adeno-associated virus particles in said sample, wherein said number of target cells infected is directly proportional to the titer of said particles in said sample, thereby determining the titer if said adeno-associated virus particles.

REFERENCES:
patent: 6566118 (2003-05-01), Atkinson et al.
patent: 6703237 (2004-03-01), Samulski et al.
Xiao Xiao et al; Production of High-Titer Recombinant Adeno-Associated Virus Vectors in the Absence of Helper Adenovirus; Journal of Virology, Mar. 1998, p. 2224-2232.
T. Matsushita et al; Adeno-associated virus vectors can be efficiently produced without helper virus; Gene Therapy (1998) 5, 938-945.

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