Method and kits for preparing multicomponent nucleic acid...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C435S091100, C435S091200, C435S254200, C435S320100, C536S022100, C536S024300, C536S024330, C536S025300

Reexamination Certificate

active

10280261

ABSTRACT:
The invention provides a highly efficient, rapid, and cost effective method of linking nucleic acid components in a predetermined order to produce a nucleic acid multicomponent construct. The invention further provides nucleic acid components, each nucleic acid component comprising a double stranded nucleic acid molecule having at least one single stranded 5′ or 3′ terminal sequence, the terminal sequence having sufficient complementarity to either a terminal sequence in a separate nucleic acid component or to a sequence in a linking nucleic acid molecule so as to allow for specific annealing of complementary sequences and linkage of the components in a predetermined order. Kits containing reagents required to practice the method of the invention are also provided.

REFERENCES:
patent: 4987073 (1991-01-01), Berman et al.
patent: 5128256 (1992-07-01), Huse et al.
patent: 5137814 (1992-08-01), Rashtchian et al.
patent: 5426039 (1995-06-01), Wallace et al.
patent: 5470955 (1995-11-01), Craig
patent: 5962255 (1999-10-01), Griffiths et al.
patent: 6365344 (2002-04-01), Nolan et al.
patent: 6495318 (2002-12-01), Harney
patent: WO 93/20242 (1993-10-01), None
Watson et al. 1992 Recombinant DNA, Second Ed. pp. 206-209).
Goodchild Bioconjugate Chemistry, vol. 1, pp. 165-187, May/Jun. 1990.
Stratagene Catalog, p. 39, 1988.
Aslanidis C. and de Jong P.J. (1990) “Ligation-independent cloning of PCR products (LIC-PCR)”,Nucleic Acids Research 18(20):6069-6074.
Belev, T.N. et al (1991) “A Fully Modular Vector System for the Optimization of Gene Expression inEscherichia coli” Plasmid 2:147-150.
Brosius J. (1989) “Laboratory Methods Superpolylinkers in Cloning and Expression Vectors”,DNA 8(10):759-777.
Clark J.M. (1988) “Novel non-templated nucleotide addition reactions catalyzed by procaryotic and eucaryotic DNA polymerases”, Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, NCNucleic Acid Research 16(20):9677-9685.
Denis, F. and Brzezinski, R. (1992) “A versatile shuttle consmid vector for use inEscherichia coliand actinomycetes”Gene 111:115-118.
Gibson, T. et al (1987) “Loristó, a cosmid vector with BarnHl, Notl, Scal and Hindlll cloning sites and altered neomycin phosphotransferase gene expression”Gene 53:283-286.
Goeddel, D. et al (1992) “Gene Expression Technology”Method in Enzymology 185:512-527.
Goodchild,Bioconjugate Chemistry 1(3):165-187, 1990.
Marchuk D. et al (1990) “Construction of T-vectors, a rapid and general system for direct cloning of unmodified PCR products”,Nucleic Acids Research 19(5):1154.
MacNeil D.J. et al (1992) “Analysis ofStreptomyces avermitilisgenes required for avermectin biosynthesis utilizing a novel integration vetor”,Gene 111:61-68.
Scharf S.J. et al (1986) “Direct Cloning and Sequence Analysis of Enzymatically Amplified Genomic Sequences”,Science 233(4768):1076-1078.
Watson, J.D. et al (1994) “Recombinant DNA, Second Edition”.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Method and kits for preparing multicomponent nucleic acid... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method and kits for preparing multicomponent nucleic acid..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method and kits for preparing multicomponent nucleic acid... will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3721245

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.