Method and kits for multiplex hybridization assays

Details Method and kits for multiplex hybridization assays Method and kits for multiplex hybridization assays

2008-05-06

2008-05-06

Whisenant, Ethan (Department: 1634)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C536S023100, C536S024300

Reexamination Certificate

active

11152460

ABSTRACT:
The invention provides a method for genotyping interfering polymorphic loci in a target polynucleotide, such as a strand of genomic DNA, in a multiplex hybridization-based assay. The invention also provides nucleic acid standards for validating the performance of such hybridization-based assays. In one aspect, the method of the invention is carried out by providing for each interfering polymorphic locus one or more probes so that at least one probe is capable of forming a perfectly match duplex at the locus regardless of the characteristic sequence of an adjacent polymorphism.

REFERENCES:
patent: 5445934 (1995-08-01), Fodor
patent: 5861242 (1999-01-01), Chee
patent: 5981174 (1999-11-01), Wolf
patent: 6355431 (2002-03-01), Chee
patent: 6448013 (2002-09-01), Arnold
patent: 6753141 (2004-06-01), Bernard
patent: 6858412 (2005-02-01), Willis
patent: 2003/0003490 (2003-01-01), Fan
patent: 2003/0104436 (2003-06-01), Morris
patent: 2005/0250147 (2005-11-01), Macevicz
patent: 2006/0019304 (2006-01-01), Hardenbol et al.
US 5,962,233, 10/1999, Livak (withdrawn)
The Stratagene Catalog p. 39 (1988).
Shumaker et al., Mutation detection by solid phase primer extension. Human Mutation 7 : 346-354 (1996).
Saiki et al. Analysis of enzymatically amplified -globin and HLA-DQ DNA with allele-specific oligonucleotide probes. Nature 324 : 163-166 (1986).
Wen et al, “Rapid detection of the known SNPs of CYP2C9 using oligonucleotide microarray,” World Journal of Gastroenterology, 9: 1342-1346 (2003).
Daly, “Pharmacogenetics of the major polymorphic metabolizing enzymes,” Fundamental & Clinical Pharmacology, 17: 27-41 (2003).
Landi et al, “Evaluation of a microarray for genotyping polymorphisms related to xenobiotic metabolism and DNA repair,” BioTechniques, 35: 816-827 (2003).
Chou et al, “Comparison of two CYP2D6 genotyping methods and assessment of genotype-phenotype relationships,” Clinical Chemistry, 49: 542-551 (2003).
Linder et al, “Pharmacogenetics: a laboratory tool for optimizing therapeutic efficiency,” Clinical Chemistry, 43: 254-266 (1997).
Greenspoon et al, “Validation and implementation of the PowerPlex 16 BIO system STR multiplex for forensic casework,” J. Forensic Sci., 49: 71-80 (2004).
Hardenbol et al, “Multiplexed genotyping with sequence-tagged molecular inversion probes,” Nature Biotechnology, 21: 673-678 (2003).

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