Method and kits for determining messenger RNA molecules in a sam

Details Method and kits for determining messenger RNA molecules in a sam Method and kits for determining messenger RNA molecules in a sam

1996-07-22

1999-09-21

Horlick, Kenneth R.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 911, 435 912, 435 9151, 536 221, C12P 1934, C07H 1900

Patent

active

059552719

ABSTRACT:
The invention involves methods and kits useful in determining specific mRNA molecules. The methods can be carried in a single reaction vessel, in that both reverse transcriptase and amplification can be carried out in the same, heat stable reaction vessel.

REFERENCES:
patent: 5354657 (1994-10-01), Holtke
patent: 5424189 (1995-06-01), Oberst
Reyes-Engel, et al. "Direct Quantitation of specific mRNA using a selected biotinylated oligonucleotide by free solution capillary electrophoresis" Nucl. Acids Res. 21(s): 759-60 (1993).
Cuddy, et al., "RT-PCR with affinity captured mRNA" Nucl. Acids Res. 21(9): 2281(1993).
Derwent AN 94-337443 (corresponding to JP 06 261800) Sep. 20, 1994.

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