Method and kits for detection of fragile X specific, GC-rich DNA

Details Method and kits for detection of fragile X specific, GC-rich DNA Method and kits for detection of fragile X specific, GC-rich DNA

1995-06-07

1997-08-19

Houtteman, Scott W.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

536 2433, C12P 1934

Patent

active

056587643

ABSTRACT:
A method is provided for amplifying and detecting specific GC-rich nucleic acid sequences contained in a nucleic acid or in a mixture of nucleic acids, which includes treating a separate nucleic acid containing the specific sequence with a molar excess of primers and a polymerase and extending the primers in the presence of dATP, dCTP, TTP, and an analogue of dGTP. In one application of the present invention, individuals who are carriers for, or afflicted by, the fragile X syndrome are detected.

REFERENCES:
patent: 4965188 (1990-10-01), Mullis et al.
patent: 5091310 (1992-02-01), Innis
Innis et al., "PCR with 7-Deaze-2' deoxyguanosine triphosphate," in PCR Protocols, Ed. by Innis 1990.
Kremer et al., Science 252:1711-1714 Jun. 21, 1991.
1988 Stratagene Catalog, p. 39.

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