Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1995-03-28
1998-08-11
Marschel, Ardin H.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 911, 435 912, 435810, 436501, 536 231, 536 241, 536 243, 536 2431, 536 2432, 536 2433, 935 77, 935 78, 935 88, C12Q 168, C12P 1934
Patent
active
057926077
ABSTRACT:
The present invention involves methods of improving LCR and PCR amplification schemes by modifying at least one probe/primer end so that the probability of the probe/prirner contributing to spurious signal development is greatly reduced. Only after specific hybridization of the modified probe/primer with true target, are the modified ends "corrected" in a target dependent fashion to allow participation of the probe/primer in the enzymatic assembly reaction. Specific modifications depend on whether the assembly is done by ligation (as in LCR) or by extension/elongation (as in PCR).
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Backman Keith C.
Bond Sheila B.
Carrino John J.
Laffler Thomas G.
Abbott Laboratories
Brainard Thomas D.
Marschel Ardin H.
Yasger Paul D.
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