Method and kit for the manufacture of separation gels

Details Method and kit for the manufacture of separation gels Method and kit for the manufacture of separation gels

2006-05-30

2006-05-30

Nguyen, Nam (Department: 1753)

Chemistry: electrical and wave energy

Processes and products

Electrophoresis or electro-osmosis processes and electrolyte...

C204S470000

Reexamination Certificate

active

07052590

ABSTRACT:
A method for the manufacture of a separation gel, which comprises the steps: (i) providing a polymerization mixture containing a first monomer (I) having a polymerizable unsaturated structure and a second monomer (II) comprising two or more polymerizable unsaturated structures and an initiator, and (ii) polymerizing the mixture. The method is characterized in that monomer (I) is an N-vinyl carboxamide; and that (a) the initiating system for the polymerization comprises a UV photoinitiator and polymerization is initiated by UV irradiating the mixture at a wave-length appropriate for the initiator, and/or (b) preselecting monomer (I) and monomer (II) to have comparable reactivities to become incorporated into the growing polymer. A kit for use in the production of an electrophoretic gel is also described. The kit comprises: (a) a monomer (I) carrying an N-vinyl carboxamide group; (b) a monomer (II) carrying a polymerizable unsaturated structure; and (c) an UV initiator compound, a buffer and/or a denaturing agent; said monomers (I and II) being selected to provide comparable reactivities.

REFERENCES:
patent: 3948743 (1976-04-01), Monthony et al.
patent: 4094832 (1978-06-01), Soderberg
patent: 4721734 (1988-01-01), Gehlhaus et al.
patent: 5290411 (1994-03-01), Zewert et al.
patent: 6117293 (2000-09-01), Zhang et al.
patent: 0 087 995 (1983-09-01), None
patent: 08015222 (1996-01-01), None
patent: WO 97/26071 (1997-07-01), None
patent: WO 98/28352 (1998-07-01), None
“Applications: Free Radical Initiators” Brochure from Aldrich. pp. 5-18.
Haimo, KK: “N-Vinylcarboxamide-type polymer gels for electrophoresis, their preparation, and an electrophoresis method” STN International, File CAPLUS, CAPLUS accession No. 1996:231562, Document No. 124:283711 JP,A2,08015222,19960119.
Akashi, M., et al.: “Novel hydrogels for electrophoresis” STN International, File CAPLUS, CAPLUS accession No. 1995:136662, Document No. 122:182485 Kuromatogurafi (1994), 15(2), 108-9.
Akashi, M., et al.: “Capillary electrophoresis apparatus” STN International, File CAPLUS, CAPLUS accession No. 1997:614432, Document No. 127:230346 JP,A2,09236580, 19970909.
Showa Denko KK: “Preparation of N-vinylcarboxylic acid amide-based copolymers in low cost” STN International, File CAPLUS, CAPLUS accession No. 1997:279138, Document No. 126:277907 JP,A2,09059309,19970304.

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