Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1985-12-30
1988-07-12
Warden, Robert J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435810, 435883, 436514, 436515, 436807, 436808, 436809, 436828, G01N 3353, G01N 33558, C12R 1445
Patent
active
047570024
ABSTRACT:
A radial immunodiffusion enzyme assay method for the simple estimation of immunoglobulins in humans and other animals, Agar test plates are provided including an underlying adherent coating of Staphylococcal Protein A antigen. Blood or blood serum samples from animals to be tested are placed in wells punched in the agar layer and allowed to incubate overnight. The agar gel is then removed. The resulting Protein A antigen layer with bound immunoglobulins from the samples is reacted with enzyme conjugated anti-immunoglobulin or enzyme conjugated Protein A. The reaction is visualized by overlaying the bound conjugate layer with agar containing a color producing enzyme substrate. The diameters of resulting colored zones permit estimation of total immunoglobulins. Methods of preparing Protein A antigen coated test plates are disclosed along with testing kits for carrying out the test procedure in the field.
REFERENCES:
patent: 3947250 (1976-03-01), Pollack
patent: 3966897 (1976-06-01), Renn et al.
patent: 3995018 (1976-11-01), Sjoquist
patent: 4617262 (1986-10-01), Maxim et al.
Chemical Abstracts, vol. 83, 1975, Abstract No. 76830e, Bokhout.
Chemical Abstracts, vol. 73, 1970, Abstract No. 53957f, Heiner et al.
Chemical Abstracts, vol. 97, 1982, Abstract No. 125430z, Kajita et al.
Biological Abstracts, vol. 82, 1986, Abstract No. 23851, Schwan et al.
Am J. Vet. Res. vol. 45, No. 10 (Oct. 1984) Joo et al.
Hoffer Florina B.
Regents of the University of Minnesota
Warden Robert J.
LandOfFree
Method and kit for the estimation of serum immunoglobulin does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method and kit for the estimation of serum immunoglobulin, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method and kit for the estimation of serum immunoglobulin will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-664150