Method and kit for the detection of microorganisms

Details Method and kit for the detection of microorganisms Method and kit for the detection of microorganisms

1995-07-31

1998-02-03

Tran, Lien

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

435 4, 435 30, 435 34, 435 38, 435 39, 435810, C12M 112

Patent

active

057143439

ABSTRACT:
A method for determining the presence of microorganisms in a liquid sample. The liquid sample is first filtered through a filter having a pore size which is small enough to prevent passage of microorganisms through the filter but large enough to permit passage of any free reducing agents present in the sample whereby microorganisms present in the sample are retained on the filter. A chromogenic reagent having an oxidation potential such that the reagent can be reduced by microbial dehydrogenase and selected such that reduction of the chromogenic reagent yields a visibly colored product is then passed through the filter, and the filter is monitored for the formation of a visibly colored product indicative of the presence of microorganisms in the sample. The method can be practiced with the aid of a kit which includes a filter apparatus, a test solution for visualization of microorganisms and optionally a solution of a non-ionic alkyl glucoside-type detergent and a chaotropic agent for visualization only gram-negative bacteria.

REFERENCES:
patent: 4724204 (1988-02-01), Steinbach et al.
patent: 5081017 (1992-01-01), Longoria
patent: 5366872 (1994-11-01), Hird et al.
patent: 5366873 (1994-11-01), Eden et al.
patent: 5420017 (1995-05-01), Tuompo et al.
An 82:178725 McKinnon et al. J. Appl. Bacteriol 51(2) 1981, pp. 363-368.
An 83:210725 Herson et al, AM Water Works Assoc J. 74(10) 1982 pp. 537-539.
An 84103166 Dutton et al, Applied and Environment Microbiology 46, (6) 1983, pp. 1263-1270.

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