Method and kit for the detection of microorganisms

Details Method and kit for the detection of microorganisms Method and kit for the detection of microorganisms

1991-08-05

1995-05-30

Beisner, William H.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

435 4, 435 30, 435 34, 435 39, 435261, 435243, 435311, 435 26, 252DIG1, C12M 112, C12N 102

Patent

active

054200172

ABSTRACT:
The presence of microorganisms in a liquid sample can be determined by a method comprising the steps of (a) filtering the liquid sample through a filter having a pore size which is small enough to prevent passage of microorganisms through the filter but large enough to permit passage of any free reducing agents present in the sample whereby microorganisms present in the sample are retained on the filter; (b) passing a test solution comprising a chromogenic reagent through the filter having the retained microorganisms thereon, said chromogenic reagent having an oxidation potential such that the reagent can be reduced by microbial dehydrogenase and said chromogenic reagent being selected such that reduction of the chromogenic reagent yields a visibly colored product; and (c) monitoring the filter for the formation of a visibly colored product, wherein the formation of a visibly colored product is indicative of the presence of microorganisms in the sample. This method can be practiced with the aid of a kit which comprises a filter apparatus, a test solution for visualization of microorganisms and optionally a solution of a non-ionic alkyl glucoside-type detergent and a chaotropic agent for visualization only gram-negative bacteria.

REFERENCES:
patent: 4225669 (1980-09-01), Melnick
Michal, G. et al., "Methods in Enzymatic Analyses," vol. 1, pp. 197-232 (1983).
"Oxireductases," Histological and Histochemical Methods, Theory and Practice, 2d Ed., Chapter 16, pp. 258-287 (1990).
Baker et al., "Action of Synthetic Detergents on the Metabolism of Bacteria," J. Ex. Med., 73, pp. 249-271 (1941).
Dakay et al., "The Effect of the Synthetic Detergents on the Formazan Formation of Various Environmental Bacteria," Zbl. Bakt. Hyg., 1 Abt. Orig. B. 174 pp. 121-125 (1981).

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