Method and kit for the detection of a novel coronoavirus...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C435S975000, C435S091100, C435S091200, C536S023100

Reexamination Certificate

active

07374883

ABSTRACT:
The instant invention relates to a quantitative real time RT-PCR method for detecting Severe Acute Respiratory Syndrome-associated virus (SARS-associated virus) and to oligonucleotides and kits for detecting SARS-associated virus.

REFERENCES:
patent: 6323337 (2001-11-01), Singer et al.
patent: WO 00/58505 (2000-10-01), None
Drosten et al. (“Identification of a Novel Coronavirus in Patients with Severe Acute Respiratory Syndrome” The New England Journal of Medicine. Apr. 10, 2003. pp. 1-10).
Buck et al. (“Design Strategies and Performance of Custom DNA Sequencing Primers”) BioTechniques. Sep. 1999. 27: pp. 528-536).
Gelmini et al. (“Quantiative polymerase chain reaction-based homogeneous assay with fluorogenic probes to measure c-erbB-2 oncogene amplficiation” Clinical Chemisry. 1997. 43, 5: pp. 752-758).
Mahony et al. “Performance and Cost evaluation of one commercial and six in-house conventional and real-time reverse transcription-pcr assays for detection of severe acute respiratory syndrome coronavirus” J Clin Microbiol. Apr. 2004;42(4):1471-6.
New England Biolabs 1998/99 Catalog (NEB Catalog).
Drosten et al., “Identification of a novel coronavirus in patients with severe acute repiratory syndrome,”N. Engl. J. Med., 348(20): 1967-76 (2003).
Ksiazek et al., “A novel Coronavirus Associated with Severe Acute Respiratory Syndrome,”N. Engl. J. Med., 348:1953-1966 (2003).
Marra et al., “The genome sequence of the SARS-Associated Coronavirus,”Science300:1399-1404 (2003).
Peiris et al., “Coronavirus as a possible cause of severe acute respiratory syndrome,”Lancet361:1319-1325, (2003).
Rota et al., “Characterization of a Novel Coronavirus Associated with Severe Acute Respiratory Syndrome,”Science300:1394-1399 (2003).
Ruan et al., “Comparative full-length genome sequence analysis of 14 SARS coronavirus isolates and common mutations associated with putative origins of infection,”Lancet361:1779-1785 (2003).
Yount et al., “Reverse genetics with a full-length infectious cDNA of severe acute respiratory syndrome coronavirus,”Proc. Natl. Acad. Sci., 100:12995-30000 (2003).

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