Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving luciferase
Patent
1997-11-12
1999-04-20
Railey, II, Johnny F.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving luciferase
435 4, 435 21, 435 29, 435 32, 435 34, 435975, C12Q 102, C12Q 104, C12Q 118, C12Q 166
Patent
active
058957510
ABSTRACT:
There are provided a method and a kit for testing microbial drug resistance and a method and a kit for measuring minimum inhibitory concentration for a microorganism, in which highly reliable evaluation results are obtained by preventing false evaluation based on false-resistance of microorganisms to a drug. The method of testing microbial drug sensitivity comprises inoculating and culturing a microorganism in a drug-containing medium to obtain a medium containing the microorganism morphologically transformed by the influence of the drug, adding an ATP elution agent and an ATP deletion agent to the culture to delete the eluted ATP and other ATP in the medium, and comparing the amount of ATP in the microorganism remaining in the culture with the amount of ATP in the microorganism separately cultured in a medium not containing said drug and treated in the same manner as above. The method of measuring minimum inhibitory concentration for a microorganism is carried out in the same manner as above.
REFERENCES:
"Chemical Sensitive Test Microbe Antibiotic Culture Medium Contain Chemical Determine Amount ATP Prefer Luciferin Luciferase Compare Control Amount", Derwent Publications, Ltd., London, GB, Dec. 22, 1992 (Abstract).
Saunders et al., "Metabolism and action of neplanocin A in Chinese hamster ovary cells", Biochem. Pharmacol. (1985), 34 (15), 2749-54.
Hattori Noriaki
Nakajima Moto-o
Yajitate Keiko
Kikkoman Corporation
Railey II Johnny F.
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