Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase
Patent
1988-03-02
1992-02-25
Kepplinger, Esther L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving oxidoreductase
435 29, 435259, 435810, 436 23, C12Q 130
Patent
active
050913069
ABSTRACT:
The invention provides a method for testing for the presence of catalase in a milk sample comprising combining a milk sample with a substantially catalase-free alkaline protease enzyme enriched detergent to disrupt catalase containing somatic cells present therein and release active catalase therefrom in the present of a pH buffering salt having a concentration of at least 20 mM in the resulting sample solution and providing the solution with a pH in a range of about 7.0-8.0 and thereafter testing the sample for the presence of catalase, as well as providing a test kit for detecting the presence of catalase in milk comprising a substantially catalase-free alkaline protease enzyme enriched detergent and 20 to 400 micromoles of a pH buffer alone or in combination with any other solute capable of reducing the solubility of oxygen in milk and of providing the resulting milk solution with a pH in the range of about 7.0-8.0.
REFERENCES:
patent: 3426732 (1975-12-01), Rosen et al.
patent: 4065357 (1977-12-01), Grover
patent: 4481294 (1984-11-01), Downs
Bergmeyer, Methods of Enzymatic Analysis, vol. II, pp. 70-73 (1983).
Bergmeyer, H., Methods of Enzymatic Analysis, vol. II, pp. 126 and 165-166, 1983).
Daniels, F., et al., Physical Chemistry, 3rd Edition, (John Wiley & Sons, pubisher, New York), pp. 141, (1967).
Roger Grant, Claire Grant, Grant & Hackh's Chemistry Dictionary, 5th Edition, 1987, p. 118.
Bidwell Carol E.
Kepplinger Esther L.
Yissum Research Development Company of the Hebrew University of
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