Method and kit for separation of glycoproteins

Details Method and kit for separation of glycoproteins Method and kit for separation of glycoproteins

1979-06-27

1981-05-26

Turk, Arnold

Chemistry: physical processes

Physical processes

Crystallization

23901, 23913, 260112B, 422 61, G01N 3366, G01N 3372

Patent

active

042696054

ABSTRACT:
Glycoproteins, especially glycosylated hemoglobins, are separated from non-glycosylated proteins, by complexing with a dihydroxyboryl reactive agent. The resulting complex can be separated physically from the non-glycosylated protein, making possible the determination of glycosylated hemoglobin content of lysed blood; a reactive agent and a kit are described for use in such determination and as a monitor in the control of diabetic treatment. The invention can also be used preparatively to isolate glycoproteins.

REFERENCES:
patent: 4142857 (1979-03-01), Acuff
Weith et al, Biochemistry, vol. 9, 4396-4401 (1970).
Morris et al, "Separation Methods in Biochemistry", Sir Issac Pitman & Sons, Ltd., London, (1964).
Schott et al., Biochemistry, vol. 12, 932-938, (1973).
Schott, Angew. Chem. internat. Edit., vol. 11, 824-825 (1972).
Instruction pamphlet for Quik-Sep Fast Hemoglobin Test System, Isolab, Inc., Jul. 1, 1978.

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