Chemistry: analytical and immunological testing – Involving production or treatment of antibody
Patent
1994-03-10
1996-06-18
Scheiner, Toni R.
Chemistry: analytical and immunological testing
Involving production or treatment of antibody
435 71, 435 79, 435 792, 435 793, 435 794, 435962, 436 86, 436518, 436531, 5303879, 5303891, 5303911, 5303913, G01N 3353
Patent
active
055277152
ABSTRACT:
A method of assaying a human urine sample, by measuring a concentration of pyridinium crosslinks from which the concentration of total hydrolysed pyridinoline in the sample can be determined, is disclosed. In the method, a urine sample is reacted with an anti-Pyd antibody reagent which preferably has a ratio of reactivity toward native free pyridinoline and urinary pyridinoline peptides larger than 1,000 daltons in molecular weight, of greater than 10:1. By measuring the extent of immunocomplex formed by the reaction, the concentration of total hydrolysed pyridinoline in the sample can be calculated. Also disclosed are an antibody reagent and kit which can be used in the method.
REFERENCES:
patent: 5004806 (1991-04-01), Kung
Cerelli Mary J.
Daniloff Yuri
Kung Viola T.
Zuk Robert F.
Dehlinger Peter J.
Metra Biosystems, Inc.
Powers Vincent M.
Scheiner Toni R.
Wolski Susan C.
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