Method and kit for purifying nucleic acids

Details Method and kit for purifying nucleic acids Method and kit for purifying nucleic acids

1989-11-13

1991-03-05

Wax, Robert A.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 28, 435 6, 435259, 435810, 435267, 435268, 435269, 435270, 435280, C07H 1512, C12N 106, C12N 108, O07P 4100

Patent

active

049979329

ABSTRACT:
The invention teaches a method and kit for purifying nucleic acid, such as DNA, from a sample, such as lysed cell or tissue sample. A sample is applied to an anionic exchange matrix column uniformly distributing the sample therein. The column bed is then washed with a weak ionic salt solution which is then removed. The anionic exchange material is optionally primed with an amount strong ionic salt solution which is insufficient to elute the nucleic acid from the column. The priming solution is then removed. An elution buffer is added either directly after the washing step or after the priming step. This is also a strong ionic salt solution of an ionic salt. The elution buffer removes the purified nucleic acid from the material. The method permits purification of nucleic acids without using organic solvents, and, if the priming step is used, in more concentrated form. Uniform distribution of the sample via disturbance of the matrix or column facilitates the purification.

REFERENCES:
patent: 4482482 (1984-11-01), Haff et al.
patent: 4800159 (1989-01-01), Mullis et al.
patent: 4833239 (1989-05-01), DeBonville et al.

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