Method and kit for primer based multiplex amplification of...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C435S091200

Reexamination Certificate

active

07851148

ABSTRACT:
Disclosed is a novel method for diagnosis or differential diagnosis of disease agents and secondary disease agents. The method disclosed uses a novel amplification strategy termed TemPCR to allow sensitive and specific amplification of target sequences from any disease agents and/or secondary disease agent whose nucleic acid sequence is known. The TemPCR method utilizes at least one set of target enrichment primers specific for the disease agent or secondary disease agent to be detected (present at a low concentration) and at least one pair of shared target amplification primers (present at high concentrations). At least one pair of said target enrichment primers comprises a binding sequence for the target amplification primers. Therefore, the use of the TemPCR method allows multiplex amplification reactions to be carried out without the need for empirical optimization of the multiplex amplification parameters. Methods for nucleic acid isolation and the detection of the target sequences are also disclosed for use with the TemPCR method.

REFERENCES:
patent: 4683202 (1987-07-01), Mullis
patent: 5194300 (1993-03-01), Cheung
patent: 5314809 (1994-05-01), Erlich et al.
patent: 5340728 (1994-08-01), Grosz et al.
patent: 5422252 (1995-06-01), Walker et al.
patent: 5624825 (1997-04-01), Walker et al.
patent: 5811235 (1998-09-01), Jeffreys
patent: 5882856 (1999-03-01), Shuber
patent: 6168917 (2001-01-01), Kilpatrick
patent: 6509157 (2003-01-01), Martinez
patent: 6566067 (2003-05-01), Malo
patent: 6605451 (2003-08-01), Marmaro et al.
patent: 6737253 (2004-05-01), Tillet
patent: 7262030 (2007-08-01), Chen
patent: 2003/0096277 (2003-05-01), Chen
patent: 2003/0104459 (2003-06-01), Faham et al.
patent: 2004/0091879 (2004-05-01), Nolan et al.
patent: 2005/0175996 (2005-08-01), Chen
patent: 2006/0035222 (2006-02-01), Rudi et al.
patent: 91112959.1 (1991-08-01), None
patent: 94305400.7 (1994-07-01), None
patent: WO 0194634 (2001-12-01), None
patent: WO 03060159 (2003-07-01), None
patent: WO 03066897 (2003-08-01), None
Elnifro et al., Multiplex PCR: Optimization and Application in Diagnostic Virology, Clinical Microbiology Reviews, Oct. 2000, p. 559-570.
Qiagen, Taq PCR Handbook , Mar. 2002, pp. 1-40.
Archard et al., Characterization of Coxsackie B Virus RNA in Myocardium From Patients With Dilated Cardiomyopathy by Nucleotide Sequencing of Reverse Transcription-Nested Polymerase Chain Reaction Products, Human Pathology vol. 29, No. 6 (Jun. 1998), pp. 578-584.

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