Method and kit for performing nucleic acid hybridization assays

Details Method and kit for performing nucleic acid hybridization assays Method and kit for performing nucleic acid hybridization assays

1995-04-27

1997-06-24

Zitomer, Stephanie W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

935 77, 935 78, C12Q 168

Patent

active

056416300

ABSTRACT:
A method and a kit for the isolation and quantitative detection of a selected target nucleic acid sequence from solution employing two probes. A first probe is complementary to one portion of the target and is covalently attached to a first complexing agent (e.g., either an antigen or an antibody). The second probe is complementary to a different portion of the target and is associated with a reporter group. Following hybridization of the target and two probes in solution, a solid support coated with a second complexing agent (i.e., a corresponding antibody or antigen) capable of binding to the first complexing agent on the first probe is employed to immobilize the target-probe hybrid complex.
A plurality of types of first probes may be used. Each type is attached to the same sort of complexing agent but each includes a nucleic acid sequence which is complementary to a different portion of the target.

REFERENCES:
patent: 4486539 (1984-12-01), Ranki et al.
patent: 4766062 (1988-08-01), Diamond et al.
patent: 5030557 (1991-07-01), Hogan et al.
Matthews et al., Anal. Biochem. 169:1-25 (1988).
Langer et al. PNAS (USA) 78(11):6633-6637 (Nov. 1981).
Ranki et al. Gene 21:77-85 (1983).

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