Method and kit for making CDNA library

Details Method and kit for making CDNA library Method and kit for making CDNA library

1995-03-17

1997-05-13

Elliott, George C.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 9151, 536 2433, C12P 1934, C07H 2104

Patent

active

056291795

ABSTRACT:
A method for forming directionally clonable randomly primed cDNA molecules includes the step of priming first strand synthesis using a set of first strand cDNA primers having the sequence 5'-XXNNNNNN-3' where XX is a constant dinucleotide pair across the set and NNNNNN is a random hexanucleotide, the set including primers representing all random hexanucleotides.

REFERENCES:
patent: 5075227 (1991-12-01), Hagen
Meissner, et al., "Bacteriophage .lambda. cloning system for the construction of directional cDNA libraries," Proc. Natl. Acad. Sci. USA 84:4171-4175 (1987).
Palazzolo et al., "A family of lambda phage cDNA cloning vectors, .lambda.SWAJ, allowing the amplification of RNA seqences, " Gene 32:197-206 (1987).
Palazzolo, et al., "Phage lambda cDNA cloning vectors for subtractive hybridization, fusion-protein synthesis and Cre-loxP automatic plasmid subcloning," Gene 88:25-36 (1990).

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