Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Patent
1994-05-19
1997-06-17
Budens, Robert D.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
435 71, C12Q 170, G01N 3353
Patent
active
056395981
ABSTRACT:
Human and simian immunodeficiency viruses (HIV/SIVs) contain, in addition to the canonical gag/pol/env genes, additional small open reading frames (ORFs) encoding gene products, including the 96-amino acid 15-kDa virion-associated HIV-1 Vpr gene product. Vpr functions as a regulator of cellular processes related to HIV replication. A biologically active recombinant HIV-1 Vpr protein was employed as a ligand to identify its cognate cellular target(s). A novel 41-kDa cytosolic viral protein R interacting protein, designated Rip-1, was identified using the recited assay. Rip-1 displays a wide-tissue distribution, including relevant targets of HIV infection. HIV-1 Vpr induced nuclear translocation of Rip-1. This invention provides novel biochemical reagents and methods that will facilitate the identification of antiviral agents.
REFERENCES:
patent: 4296206 (1981-10-01), Simons, Jr.
patent: 4386085 (1983-05-01), Teutsch et al.
patent: 4447424 (1984-05-01), Teutsch et al.
patent: 4477445 (1984-10-01), Philibert et al.
patent: 4519946 (1985-05-01), Teutsch et al.
patent: 4540686 (1985-09-01), Philibert et al.
patent: 4547493 (1985-10-01), Teutsch et al.
patent: 4634695 (1987-01-01), Torelli et al.
patent: 4634696 (1987-01-01), Teutsch et al.
patent: 4753932 (1988-06-01), Teutsch et al.
patent: 4774236 (1988-09-01), Cook et al.
patent: 4814327 (1989-03-01), Ottow et al.
patent: 4829060 (1989-05-01), Ottow et al.
patent: 4861763 (1989-08-01), Cook et al.
patent: 4912097 (1990-03-01), Teutsch et al.
patent: 4943566 (1990-07-01), Nedelec et al.
patent: 4954490 (1990-09-01), Cook et al.
patent: 4978657 (1990-12-01), Teutsch et al.
patent: 5006518 (1991-04-01), Moguilewsky et al.
patent: 5011829 (1991-04-01), Hirsch et al.
patent: 5043322 (1991-08-01), Teutsch et al.
patent: 5064822 (1991-11-01), Philibert et al.
patent: 5073548 (1991-12-01), Cook et al.
patent: 5089488 (1992-02-01), Ottow et al.
patent: 5089635 (1992-02-01), Neff et al.
patent: 5093507 (1992-03-01), Scheidges et al.
patent: 5095010 (1992-03-01), Elger et al.
patent: 5095129 (1992-03-01), Ottow et al.
patent: 5132299 (1992-07-01), Ottow et al.
patent: 5166146 (1992-11-01), Moguilewsky et al.
patent: 5276023 (1994-01-01), Moguilewsky et al.
patent: 5298429 (1994-03-01), Evans et al.
patent: 5436128 (1995-07-01), Harpold et al.
Rogel et al., 1995, J. Virol. 69:882-888.
Zhao et al., 1994, J. Biol. Chem. 269:15577-15582.
Evans, R., 1988, Science 240 889-895.
Beato, M., 1989, Cell 56:335-344.
Storrie and Madden, 1990, Methods Enzymol. 182:203-225.
Levy, J., 1993, Microbiol. Rev. 57:183-289.
Holland et al., 1992, Curr. Topics Microbiol. Immunol. 176:1-20.
Heinzinger, N. et al., "The Vpr Protein of Human Immunodeficiency Virus Type 1 Influences Nuclear Localization of Viral Nucleic Acids in Nondividing Host Cells", PNAS USA 1994, 91, 7311-7315.
Refaeli, Y. et al., "The Glucocorticoid Receptor Type II Complex is Target of the HIV-1 Vpr Gene Product", PNAS USA 1995, 92, 3621-3625.
Zhao et al., "Biochemical Mechanism of HIV-1 Vpr Function", Journal of Biological Chemistry 269:15577-15582.
Valentin, et al. "In Vitro Maturation of Mononuclear Phagocytes And Susceptibility to HIV-1 Infection" J. AIDS 4:751-759 (1991).
Granner et al. [80] "Tyrosine Aminotransferase (Rat Liver)", Meth. Enzymol. 15:633-637 (1970).
Lefkowitz et al. "Isolation of Lymphocytopathic Retroviruses From San Francisco Patients With AIDS", Science 225:840-842 (1984).
Roulston et al. "Induction of Monocytic Differentiation And NF-.kappa.B-like Activities By Human Immundeficiency Virus 1 Infection Of Myelomonoblastic Cells", J. Exp. Med. 175:751-763 (1992).
Ogawa et al. "Mutational Analysis Of The Human Immunodeficiency Virus vpr Open Reading Frame", J. Virol. 63:4110-4114 (1989).
Dedera et al. "Viral Protein R of Human Immunodeficiency Virus Types 1 and 2 Is Dispensable For Replication And Cytopathogenicity In Lymphoid Cells", J. Virol. 63:3205-3208 (1989).
Levy et al., "Induction Of Cell Differentiation By Human Immunodeficiency Virus 1 vpr", Cell 72:541 (1993).
Rose et al., "Frequent Identification Of HIV-1 DNA In Bronchoalveolar Lavage Cells Obtained From Individuals With The Acquired Immunodeficiency Syndrome", Am. Rev. Respir. Dis. 143:850-854 (1986).
Gallo et al., "Frequent Detection And Isolation of Cytopathic Retroviruses (HTLV-III) Fron Patients With AIDS And At Risk For AIDS", Science 224:500-503 (1984).
Griffin et al., "Activation Of HIV Gene Expression During Monocyte Differentiation By Induction Of NF-.kappa.B", Nature 339:70-73 (1988).
Zack et al., "HIV-1 Production From Infected Peripheral Blood T Cells After HTLV-1 Induced Mitogenic Stimulation", Science 240:1026-1029 (1988).
Rich et al., "Increased Susceptibility Of Differentiated Mononuclear Phagocytes To Productive Infection With Human Immunodeficiency Virus-1 (HIV-1)", J. Clin. Invest. 89:176-183 (1992).
Schuitemaker et al., "Biological Phenotype Of Human Immunodeficiency Virus Type 1 Clones At Different Stages Of Infection: Progression Of Disease Is Associated With A Shift From Monocytotropic To T-Cell-Tropic Virus Populations", J. Virol. 66:1354-1360 (1992).
Shibata et al., "Mutational Analysis Of The Human Immunodeficiency Virus Type 2 (HIV-2) Genome In Relation To HIV-1 and Simian Immunodeficiency Virus SIV", J. Virol. 64:742-747 (1990).
Li et al., "Human Immunodeficiency Virus Type 1 DNA Synthesis, Integration, And Efficent Viral Replication In Growth-Arrested T Cells", J. Virol. 67:3969-3977.
Chantal-Petit et al., "Human Immunodeficiency Virus Infection Down-Regulates HLA Class II Expression And Induces Differentiation In Promonocytic U937 Cells", J. Clin. Invest. 79:1883-1889 (1987).
Cohen et al., "Human Immunodeficiency Virus vpr Product Is A Virion-Associated Regulatory Protein", J. Virol. 64:3097-3099 (1990).
Westervelt et al., "Dual Regulation Of Silent And Productive Infection In Monocytes By Distinct Human Immunodeficiency Virus Type 1 Determinants", J. Virol. 66:3925-3931 (1992).
Yu et al., "Open Reading Frame vpr Of Simian Immunodeficiency Virus Encodes A Virion-Associated Protein", J. Virol. 64:5688-5693 (1990).
Hattori et al., "The Human Immunodeficiency Virus Type 2 vpr Gene Is Essential For Productive Infection Of Human Macrophages", Proc. Natl. Acad. Sci. USA 87:8080-8084 (1990).
Salahuddin, S.Z. et al., Blood, 68:281 (1986).
Myers, G. et al., AIDS Res. Hum. Retrovir. 8:373 (1992).
Wong-Staal, F. et al., AIDS Res. Hum. Retroviruses 3:33-39 (1987).
Yuan, X. et al., AIDS Res. Hum. Retroviruses 6:1265-1271 (1990).
Agarwal, M.K. et al. "Glucocorticoid Antagonists" FEBS Letters, 217:221-226 (1987).
Sambrook et al. "Molecular Cloning: A Laboratory Manual", Second Edition Cold Spring Harbor Press (1989).
Voller et al., "Immunoassays for the 80's", Eds. University Park (1981).
Work, T.S. et al. "Laboratory Techniques And Biochemistry In Molecular Biology", North Holland Publishing Company, N.Y. (1978).
Wide, "Radioimmune Assay Method", Kirkham, Ed., E. & S. Livingstone, Edinburg pp. 199-206 (1970).
Myers, G., et al., "Human Retroviruses And AIDS, 1991, A Compilation And Analysis Of Nucleic Acid And Amino Acid Sequences", Division of AIDS, National Institute of Allergy and Infectious Diseases, published by Theoretical Biology and Biophysicis Group, Los Alamos National Laboratory, Los Alamos NM.
Levy David N.
Refaeli Yosef
Weiner David B.
Budens Robert D.
Parkin Jeffrey S.
The Trustees of the University of Pennsylvania
LandOfFree
Method and kit for identification of antiviral agents capable of does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method and kit for identification of antiviral agents capable of, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method and kit for identification of antiviral agents capable of will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2156913