Chemistry: molecular biology and microbiology – Kit
Patent
1999-10-19
2000-11-21
Leary, Louise N.
Chemistry: molecular biology and microbiology
Kit
530412, 530422, 435962, 435 71, C07K 100, A23J 100, G01N 3353
Patent
active
061501727
ABSTRACT:
A method for extracting prion protein from a biological material, e.g., an animal tissue or product. In a specific example, abnormal prion protein is extracted from homogenized sheep brain with hexafluoro-2-propanol. The hexafluoro-2-propanol is separated from the aqueous brain preparation by increasing the ionic strength of the aqueous solution. Prion protein in the organic extract can be further purified, or the extract can be tested, e.g., by immunoassay, for the presence of prion protein, and more particularly abnormal prion protein. The extraction process permits testing for the presence of abnormal prior protein, e.g., for diagnosis of transmissible spongiform encephalopathies (TSE).
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Safar et al, Protein Science, V.2(12), p2206-16 (Abstract Only) 1993.
Schmerr, Mary Jo, et al., "Purification of the Prion Protein from Sheep Brain Using Hydrophilic Interaction Chromatography", ABRF'98, Program & Abstracts from Genomes to Function-Technical Challenges of the Post Genome Era, Mar. 21-24, 1998, San Diego, Ca.
Schmerr, Mary Jo, et al., "Capillary Isoelectric Focusing of the Scrapie Prion Protein", Journal of Chromatography A, 802, 1997, pp. 135-141.
Schmerr, Mary Jo, et al., "A Diagnostic Test for Scrapie Infected Sheep using a Capillary Electrophoresis Immunoassay with Fluorescent Labeled Peptides", Electrophoresis, 1998, 19, pp. 409-414.
Schmerr, Mary Jo, et al., "Use of Capillary Electrophories and Fluorescent Labeled Peptides to Detect the Abnormal Prion in the Blood of Animals that are Infected with a Transmissible Spongiform Encephalopathy", Journal of Chromatography A, 853, 1999, pp. 207-214.
Bolton, David C., et al., "Molecular Characteristics of the Major Scrapie Prion Protein", Biochemistry, 1984, 23, pp. 5898-5899.
Alpert Andrew J.
Schmerr Mary Jo
Fado John D.
Leary Louise N.
Ribando Curtis P.
Silverstein M. Howard
The United States of America as represented by the Secretary of
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