Method and kit for enzymatically determining the pH of a specime

Details Method and kit for enzymatically determining the pH of a specime Method and kit for enzymatically determining the pH of a specime

1995-03-06

1996-10-22

Czaja, Donald E.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

435 10, 435 12, 435 14, 435 25, 435 28, C12Q 162, C12Q 158, C12Q 154, C12Q 126

Patent

active

055675816

ABSTRACT:
The present invention concerns a method of enzymatically determining the pH of a specimen (e.g., a solution or a biological fluid) and a kit for conducting the method. The present method involves mixing (1) a specimen with (2) an enzyme and (3) one or more substrates for the enzyme in a buffered solution having a pH effective to provide a direct proportional relationship between the activity of the enzyme and the pH of the specimen; determining the activity of the enzyme; and correlating the activity of the enzyme to the pH of the specimen. Each of the sample, the enzyme, the substrate and the buffered solution is present in an amount effective to provide the direct proportional relationship between the activity of the enzyme and the pH of the specimen. The present kit contains an enzyme, one or more substrates for the enzyme present in an amount effective to determine the enzyme activity, and a buffered solution having a pH effective to provide a direct proportional relationship between the activity of the enzyme and the pH of the specimen. The present invention is particularly applicable to detecting adulteration of a urine specimen.

REFERENCES:
patent: 3875011 (1975-04-01), Rubenstein et al.
patent: 4120755 (1978-10-01), Pierre et al.
patent: 4190496 (1980-02-01), Rubenstein
patent: 4447527 (1984-05-01), Monte et al.
patent: 4621048 (1986-11-01), Ashihara et al.
patent: 4666855 (1987-05-01), Yang et al.
patent: 5238809 (1993-08-01), Wolfbeis
The Toyobo Manual, Section I, Enzymes, selected pages (undated).
Cody et al. Journal of Analytical Toxicology vol. 13 (1989) (pp. 217-284).

Affiliated with

Also associated with

Rating

Method and kit for enzymatically determining the pH of a specime does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method and kit for enzymatically determining the pH of a specime, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method and kit for enzymatically determining the pH of a specime will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2357969