Method and kit for enzymatically determining the pH of a specime

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or...

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435 10, 435 11, 435 12, 435 14, 435 28, C12Q 100

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active

055958672

ABSTRACT:
The present invention concerns a method of enzymatically determining the pH of a specimen (e.g., a solution or a biological fluid) and a kit for conducting the method. The present method involves mixing (1) a specimen with (2) an enzyme and (3) one or more substrates for the enzyme in a buffered solution having a pH effective to provide a direct proportional relationship between the activity of the enzyme and the pH of the specimen; determining the activity of the enzyme; and correlating the activity of the enzyme to the pH of the specimen. Each of the sample, the enzyme, the substrate and the buffered solution is present in an amount effective to provide the direct proportional relationship between the activity of the enzyme and the pH of the specimen. The present kit contains an enzyme, one or more substrates for the enzyme present in an amount effective to determine the enzyme activity, and a buffered solution having a pH effective to provide a direct proportional relationship between the activity of the enzyme and the pH of the specimen. The present invention is particularly applicable to detecting adulteration of a urine specimen.

REFERENCES:
patent: 4190496 (1980-02-01), Rubenstein

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