Method and kit for diagnosis of pseudorabies

Details Method and kit for diagnosis of pseudorabies Method and kit for diagnosis of pseudorabies

1983-06-13

1985-12-31

Nucker, Christine M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 7, 435810, 436514, 436515, 436807, 436808, 436809, C12Q 170, G01N 3354, B65D 7100

Patent

active

045621470

ABSTRACT:
A radial immunodiffusion enzyme assay method for the simple testing of pseudorabies antibodies in swine and other animals. Agar test plates are provided including an underlying adherent coating of solubilized non-infectious swine pseudorabies antigen. Blood or blood serum samples from affected animals are placed in wells punched in the agar layer and allowed to incubate overnight. The agar gel is then removed. The resulting antigen layer with bound antibodies from the samples is washed and reacted with enzyme conjugated anti-swine immunoglobulin. The reaction is visualized by overlaying the bound conjugate layer with agar containing a color producing enzyme substrate. The diameters of resulting colored zones are correlated with the titers obtained by the official virus neutralization test. Methods of preparing antigen and antigen coated test plates are disclosed along with testing kits for carrying out the test procedure in the field.

REFERENCES:
patent: 4470967 (1984-09-01), Gough et al.
Yen et al., Chem. Abstracts, 97(1982), 21812j.

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