Method and kit for diagnosis of diseases

Details Method and kit for diagnosis of diseases Method and kit for diagnosis of diseases

1990-07-20

1992-10-20

Kepplinger, Esther L.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 79, 435 34, 435 39, 435 33, 435 731, 435 732, 435 40, 435288, 422 58, 436514, 436515, 436165, 436809, C12Q 100, C12Q 104, C12Q 106, C12Q 108

Patent

active

051569485

ABSTRACT:
An improved simplified enzyme-linked immunosorbent assay (ELISA) for the diagnosis of viral, fungal, bacterial and parasitic diseases. The diagnostic test is designed for use in non-laboratory settings where the usual equipment and supplies, including running water, may not be available. Collected blood samples are applied to a previously prepared test plate having an antigen coating covered by a layer of agar. After incubation to cause antibodies from the samples to bind to the antigen coating, the agar layer is stripped off and a conjugate is applied in the form of a species specific enzyme-linked anti-immunoglobulin. After incubation to cause this conjugate to bind to the antibody-antigen coating, a previously prepared chromogen agar paper (CAP) impregnated with an enzyme substrate and acceptor, if needed, in agar is applied over the conjugate. After incubation to cause a color reaction, the results are read and interpreted in comparison with known standards. The invention includes a diagnostic kit for field testing.

REFERENCES:
patent: 3416998 (1968-12-01), Streitfeld
patent: 3482943 (1969-12-01), Csizmas et al.
patent: 4275149 (1981-06-01), Litman et al.
patent: 4299916 (1981-11-01), Litman et al.
patent: 4562147 (1985-12-01), Joo

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