Method and kit for determining total digoxin levels involving ag

Details Method and kit for determining total digoxin levels involving ag Method and kit for determining total digoxin levels involving ag

1985-04-22

1987-10-06

Marantz, Sidney

Chemistry: analytical and immunological testing

Involving immune complex formed in liquid phase

436 63, 436172, 436177, 436537, 436546, 436808, 436815, 436825, G01N 3353, G01N 33533, G01N 33536, G01N 33542

Patent

active

046983155

ABSTRACT:
A method for determining total digoxin levels in a biological fluid sample containing digoxin-protein complex which method comprises disrupting the digoxin-protein complex by treating said sample solution with an effective amount of a dissociation agent selected from one or more members of the group consisting of a C.sub.3 -C.sub.26 saturated or unsaturated fatty acid, a C.sub.2-7 lower alkanol, quinidine and .alpha.-tocopherol so as to release the bound digoxin from serum protein, passing the treated sample solution through a centrifugal ultrafilter so as to selectively pass digoxin containing sample solution through said filter and retaining said serum proteins and determining the digoxin contents in said sample solution in a fluorescence polarization assay. A kit for carrying out the method is provided.

REFERENCES:
patent: 4056468 (1977-11-01), Breiter
patent: 4492762 (1985-01-01), Wang
Chemical Abstracts, I, 102:17014e (1985).
Chemical Abstracts, II, 102:89,585q (1985).
Scherrman, J. M. et al., Clin. Chem., 30(2), 337-338 (1984).
Pudek, M. R. et al., Clin. Chem., 29(11), 1972-1974 (1983).

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