Method and kit for determining the functional activity of protei

Details Method and kit for determining the functional activity of protei Method and kit for determining the functional activity of protei

1990-06-29

1992-09-15

Housel, James C.

Chemistry: analytical and immunological testing

Peptide, protein or amino acid

436 69, 436517, 435 72, 435 13, 435 791, 435291, 435975, 422 61, 422 73, G01N 3368, G01N 3386

Patent

active

051478056

ABSTRACT:
To determine the biological activity of protein S in a sample of human plasma, the suitably diluted sample is added to a substrate formed from plasma deficient in protein S, in which protein C is activated. The functionality of protein is evaluated by a coagulometric test using bovine thromboplastin with added calcium as the phospholipid source. Also disclosed is a kit for determining the activity of Protein S in a human plasma sample. The kit includes a first reagent which is human plasma deficient in protein S, a second reagent which contains an activator for protein C, and a third reagent which contains bovine thromboplastin.

REFERENCES:
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