Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Reexamination Certificate
2003-07-17
2010-02-09
Lam, Ann Y. (Department: 1641)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
C435S007930, C435S008000, C435S025000, C435S069100, C435S971000, C435S975000, C436S518000, C436S538000, C436S172000, C530S389200, C530S807000
Reexamination Certificate
active
07659078
ABSTRACT:
A solid-phase immunoassay for 6-keto-Prostaglandin F1α, the stable hydrolysis product of prostacyclin (Prostaglandin I2) is disclosed. Prostacyclin, a potent vasodilator with anti-platelet and anti-proliferative properties is an effective treatment for primary pulmonary hypertension and pulmonary arterial hypertension associated with scleroderma and scleroderma-like syndrome. Levels of 6-keto-Prostaglandin F1αcan be directly correlated with levels of prostacyclin. Therefore, 6-keto-Prostaglandin F1αhas become the indicator of choice to measure prostacyclin levels. The single step immunoassay for 6-keto-Prostaglandin F1αuses the bioluminescent protein, aequorin as a label. Analyte-label conjugates were constructed by linking the carboxyl group of 6-keto-Prostaglandin F1αand lysine residues of aequorin by chemical conjugation methods. The binding properties of 6-keto-Prostaglandin F1αtowards its antibody and the bioluminescent properties of aequorin are retained in the conjugate. The concentration of 6-keto-Prostaglandin F1αafter extraction from plasma shows good correlation with the concentration of 6-keto-Prostaglandin F1αobtained without prior extraction of the same plasma sample. The assay allows the measurement of 6-keto-Prostaglandin F1αdirectly in plasma without any pre-treatment of the samples, which results in a much simpler method with a faster assay time.
REFERENCES:
patent: 4604364 (1986-08-01), Kosak
patent: 5486455 (1996-01-01), Stults
patent: 5876935 (1999-03-01), Pankratz et al.
patent: 5942407 (1999-08-01), Liotta et al.
Pradelles et al., 1985. Enzyme immunoassays of eicosanoids using acetylcholine esterase as label: an alternative to radioimmunoassay. Anal. Chem. 57: 1170-1173.
Luke et al., 1992. Determination of prostaglandin metabolites in biological samples by competitive time-resolved fluoroimmunoassay. Journal Immunological Meth. 148: 217-223.
Desai et al., 2002. Determination of prostacyclin in plasma through a bioluminescent immunoassay for 6-keto-prostaglandin F1alpha: implication of dosage in patients with primary pulmonary hypertension. Anal. Chem. 74: 3892-3898.
Zatta, Paolo F., “A new bioluminescent assay for studies of protein G and protein A binding to IgG and IgM.” Journal of Biochemical and Biophysical Methods, 32, 1996, pp. 7-13.
Schramm, Wilifired., et al. “Rapid Solid Phase Immunoassay for 6-keto Prostaglandin F1αon Microplates.” Clinical Chemistry, 36/3, 1990, pp. 509-514.
Tonai, Takeharu., et al. “Enzyme Immunoassay of 6-ketoprostaglandin F1αin solid phase.” Biochimica et Biophysica Acta, 836, 1985, pp. 335-343.
Fischer, C., et al. “Simultaneous Determination of 6-oxo-prostaglandin F1αand 2,3-Dinor-6-oxo-prostaglandin F1αin Biological Fluids by Stable Isotope Dilution and Negative Ion Chemical Ionization Mass Spectrometry.” Biomedical mass Spectrometry, vol. 12, No. 8, 1985, pp. 399-405.
Maclouf, Jacques., et al. “125 I Derivatives of Prostaglandins: A Novel Approach in Prostoglandin Analysis by Radioimmunoassay.” Biochimica et Biophysica Acta, 431, 1976, pp. 139-146.
Zenno, Shuhei., et al. “Bioluminescent Immunoassay Using a Fusion Protein of Protein A and the Photoprotein Aequorin,” Biochemical and Biophysical Research Communications, vol. 171, No. 1, Aug. 31, 1990, pp. 169-174.
Galvan, Barbara., et al. “Bioluminescence Hybridization Assays Using Recombinant Aequorin. Application to the Detection of Prostate-Specific Antigen mRNA.” Analytical Chemistry, vol. 68, No. 20, Oct. 15, 1996, pp. 3545-3550.
Stults, Nancy, L.., et al. “Use of recombinant Biotinylated Aequorin in Microtiter and Membrane-Based Assays: Purification of Recombinant Apoaequorin fromEscherichia coli.” Biochemistry, 1992, vol. 31, pp. 1433-1442.
Ramanatha, Sridhar., et al. “Heterogeneous bioluminescence binding assay for an octapeptide using recombinant aequorin.” Analytica Chimica Acta, 369, 1998, pp. 181-188.
Shimimura, Osamu. “A Short Story of Aequorin.” The Biological Bulletin, vol. 189, No. 1, Aug. 1995, pp. 1-5.
Lewis, J.C., et al. “Bioluminescence and Secondary Structure Properties of Aequorin Mutants Produced for Site-Specific Conjugation and Immobilization.” Bioconjugate Chem, 2000, vol. 11, No. 1, pp. 65-70.
Grabarek, Zenon., et al. “Zero-Length Crosslinking Procedure with the Use of Active Esters.” Analytical Biochemistry, 185, 1990, pp. 131-135.
Daunert Sylvia
Deo Sapna K.
Desai Urvee
Poon Michael
Grun James L.
Lam Ann Y.
McDermott Will & Emery LLP
University of Kentucky Research Foundation
LandOfFree
Method and kit for determination of prostacyclin in plasma does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method and kit for determination of prostacyclin in plasma, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method and kit for determination of prostacyclin in plasma will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-4175972