Method and kit for determination of herpes simplex viral antigen

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage

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435 792, 435 795, 435961, 435975, 435 28, 436531, 436534, 436174, G01N 33571, G01N 33546

Patent

active

051550210

ABSTRACT:
Herpes simplex viral antigen can be readily determined by contacting a specimen containing Herpes simplex virus of herpes simplex viral-infected cells with polymeric particles which have a surface area of from about 0.1 to about 600 m.sup.2 /g. Within a few minutes of this contact, antigen which is bound to the particles is contacted with antibodies thereto so as to form an immunological complex on the particles. Bound complex is separated from uncomplexed materials, and the presence of the complex is then appropriately determined. A kit for determining herpes comprises the particles described above, a disposable test device having a microporous membrane and antibodies to herpes simplex viral antigen.

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Rossier et al, "Microcarries in combination with enzyme immunofiltration and immunofluorescence for the detection of herpes simplex virus antigens in culture". J. Clin. Microbiol. vol. 21, No. 3, pp. 335-339 (Mar. 1985).

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