Method and kit for detection of oxalate

Details Method and kit for detection of oxalate Method and kit for detection of oxalate

1994-06-20

1997-02-18

Gitomer, Ralph

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving transferase

435 791, C12Q 148, G01N 3353

Patent

active

056041110

ABSTRACT:
The subject invention concerns the novel use of formyl-CoA transferase enzyme together with oxalyl-CoA decarboxylase enzyme for the detection and measurement of oxalate in biological samples. The use of the enzyme system according to the subject invention results in the conversion of oxalate into carbon dioxide and formate. Because the production of formate is directly correlated to the concentration of oxalate present in a sample, the determination of the resulting formate concentration provides an accurate, sensitive and rapid means for detecting even low levels of oxalate.

REFERENCES:
patent: 4539118 (1985-09-01), Crider
Binette, Y., Concentration of Urinary Oxalates: Comparison of Three Methods, Ann Biochim Clin Que 24 (3) 93-96 1985.
Infantes, J., Kinetic-Enzymatic Determination of Oxalate . . . Analytica Chimica Acta 242 (1991) 179-183.
Costello J., Determination of Evolved .sup.14 CO.sub.2. . . Anal Biochem 202 337-339 (1992).

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