Method and kit for detecting porcine reproductive and...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C435S007100

Reexamination Certificate

active

07449296

ABSTRACT:
The invention provides methods, devices, and kits for the detection of a class of porcine reproductive and respiratory syndrome (PRRS) virus, or PRRSV, in a biological fluid. The invention is based in part on an agent which binds the nucleocapsid (N) protein of PRRSV. The agent may be an antibody that binds the N protein with specificity. Compositions and devices comprising the binding agent, as well as methods of using it are also provided. Also provided are methods of preparing devices and kits for the practice of the methods.

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Cancel-Triado et al (Veterinary Immunology and Immunopathology, 2004, vol. 102, pp. 249-262.
Teifke, J.P., et al. “Detection of European Porcine Reproductive and Respiratory Syndrome Virus in Porcine Alveolar Macrophages by Two-colour Immunofluorescence and In-situ Hybridization-immunohistochemisry Double Labelling”,J. Comp. Path(2001) 124:238-245.
Meulenberg, J.J. M., et al. Localization and Fine Mapping of Antigenic Sites on the Nucleocapsid Protein N of Porcine Reproductive and Respiratory Syndrome Virus with Monoclonal Antibodies,Virology(1998) 252:106-114.
Yang, L., et al. “Antigenic and genetic variations of the 15 kD nucleocapsid protein of porcine reproductive and respiratory syndrome virus isolates”,Arch Virol.(1999) 144:525-546.

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