Method and kit for detecting polyriboadenosine segments and mess

Details Method and kit for detecting polyriboadenosine segments and mess Method and kit for detecting polyriboadenosine segments and mess

1985-05-02

1988-04-05

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 7, 435 17, 435 21, 435810, 436501, 536 26, 536 27, 536 28, 935 77, 935 82, C12Q 168, C12Q 150, C12Q 142, G01N 3353

Patent

active

047358971

ABSTRACT:
RNA such as messenger RNA is digested to nucleotide phosphates including AMP or ADP. The ATP or a byproduct of the phosphorylation, e.g., pyruvate, is detected. Exemplary enzymes used (with appropriate co-reactants and co-factors) are: (1) polynucleotide phosphorylase, pyruvate kinase and luciferase, or (2) phosphodiesterase (or RNase), myokinase, pyruvate kinase and luciferase. The phosphorylation to ATP (e.g., with pyruvate kinase) is preferably coupled with the previous (reversible) enzymatic step.

REFERENCES:
patent: 4446231 (1984-05-01), Self
Molloy, G. R. et al., Biochem, 11 No. 17:3256-3260 (1972).
Soreq, H. et al., Journ Molec. Biol. 88:233-245 (1974).
Holm-Hansen, O., et al., Methods in Enzymology, vol. LVII: pp. 73-85, (1978).
Dunn, A. R., et al., Methods in Enzymology, vol. 65, pp. 468-478 (1980).
Littauer et al., "Polynucleotide Phosphorylase" in The Enzymes, vol. XV (1982), pp. 517-553.

Affiliated with

Also associated with

Rating

Method and kit for detecting polyriboadenosine segments and mess does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method and kit for detecting polyriboadenosine segments and mess, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method and kit for detecting polyriboadenosine segments and mess will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2233435