Method and kit for detecting methicillin-resistant Staphylococcu

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 911, 435 912, 435810, 435883, 536 237, 536 2432, 536 2433, 935 1, 935 8, 935 72, 935 77, 935 78, C12Q 168, C12P 1934, C07H 2104, C12N 1500

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057028958

ABSTRACT:
A method and a kit for detecting methicillin-resistant Staphylococcus aureus which use, as primers in a gene amplification reaction, the four oligonucleotides represented by the following nucleotide sequences (1) through (4):

REFERENCES:
Ubukata, et al., Rapid Detection of the mecA Gene in Methicillin-Resistant Staphylococci by Enzymatic Detection of the Polymerase Chain Reaction Products, J. Clin. Micro. 30(7):1728-1733, Jul. 1992.
Frenay, H., et al., Discrimination of the Epidemic and Nonepidemic Methicillin Resistant Staphylococcus aureus Strains on the Basis of Protein A Gene Polymorphism, J. Clin. Micro. 32(2):846-847, Mar. 1994.
Ryffel, et al., Sequence comparison of the mecA genes isolated from methicillin-resistant Staphylococcus aureus and Staphylococcus epidermidis, Gene 94:137-138, Aug. 1990.
Uhlen, et al., Complete Sequence of the Staphylococcal Gene Encoding Protein A, J. Biol. Chem. 259(3):1695-1702, Feb. 1984.

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