Chemistry: analytical and immunological testing – Biospecific ligand binding assay – Utilizing isolate of tissue or organ as binding agent
Patent
1997-11-19
1999-10-26
Housel, James C.
Chemistry: analytical and immunological testing
Biospecific ligand binding assay
Utilizing isolate of tissue or organ as binding agent
427 213, 435 71, 435 72, 435 79, 435 792, 435 794, 435 13, 435 28, 435961, 435970, 435975, 436501, 436503, 436513, 436518, 436529, 436174, 436175, 436176, 436530, 436531, 436532, 436808, C12Q 156, C12Q 128, G01N 3353, G01N 33563
Patent
active
059727179
ABSTRACT:
A method of detecting heparin-induced antibodies to complete a diagnosis of heparin-induced thrombocytopenia (HITP) is disclosed. This method comprises the first step of attaching a glycosaminoglycan to a solid support, wherein the glycosaminoglycan is attached to the solid support only at the reducing end of the molecule (unidirectionally). Platelet factor 4 is then bound to the glycosaminoglycan forming a complex having an epitope recognizable by antibodies generated in an HITP immune response. Human blood plasma or serum from a patient suspected of having HITP is exposed to the complex and the complex is analyzed to determine if HITP-related antibodies are present. A device and kit used in performing the diagnostic assay are also disclosed.
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Aster Richard H.
Visentin Gian
Gabel Gailene R.
Housel James C.
The Blood Center Research Foundation, Inc.
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