Method and kit for confirming in vitro diagnosis of coeliac dise

Details Method and kit for confirming in vitro diagnosis of coeliac dise Method and kit for confirming in vitro diagnosis of coeliac dise

1997-07-23

1998-10-06

Duffy, Patricia

Chemistry: analytical and immunological testing

Biospecific ligand binding assay

Utilizing isolate of tissue or organ as binding agent

436513, 436518, 436519, 436547, 435 721, 435975, G01N 33567

Patent

active

058175237

ABSTRACT:
In order to diagnose the coeliac disease antiendomysial antibodies are detected through immunofluorescence techniques in culture media in which bioptic fragments of the human enteral mucosa are kept alive. The cultures of the enteral mucosa take place by dipping the bioptic fragment into nutrient liquids with or without the presence of low concentrations of peptic-tryptic digest of gliadin. The peptide fragment of gliadin containing a definite aminoacid sequence capable of production of antiendomysial antibodies to a greater extent than the PT digest, even in very little concentration, is also identified.

REFERENCES:
Ferreira et al. Endomysial antibody: is it the best screening test for coeliac disease? Gut 33:1633-1637, 1992.
Lerner et al. Immunological diagnosis of childhood coeliac disease: comparison between antigliadin, antireticulin, and antiendomysial antibodies, Clin. Exp. Immunol. 95:78-82, 1994.
Vazquez et al. Screening for asymptomatic celiac sprue in families, J. Clin. Gastroenterol. 21:130-133, 1995.
Vogelsang et al. Screening for celiac disease: a prospective study on the value of noninvasive tests, 90:394-398, 1995.
Ascher et al. A new laboratory kit for antigliadin IgA at diagnosis and follow-up of childhood celiac disease, J. Pediatric Gastroenterol. 10:443-450, 1990.
Devine et al. Screening and monitoring coeliac disease: multicentre trial of a new serum antibody test kit, Disease Markers 12:71-80, 1994.
Berger et al. Evaluation of six anti-gliadin antibody assays, J. Immunol. Methods 191:77-86, 1986.

Affiliated with

Also associated with

Rating

Method and kit for confirming in vitro diagnosis of coeliac dise does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method and kit for confirming in vitro diagnosis of coeliac dise, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method and kit for confirming in vitro diagnosis of coeliac dise will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-76353