Method and kit for calibrating a photoluminescence...

Details Method and kit for calibrating a photoluminescence... Method and kit for calibrating a photoluminescence...

2011-05-24

2011-05-24

Wallenhorst, Maureen M (Department: 1777)

Chemistry: analytical and immunological testing

Composition for standardization, calibration, simulation,...

C436S164000, C436S171000, C436S172000, C073S001010, C702S022000, C702S028000, C250S252100, C250S458100, C422S082050, C422S082080, C422S430000, C356S243100

Reexamination Certificate

active

07947502

ABSTRACT:
The invention is directed to a method and a kit for calibrating a photoluminescence measurement system, in particular a fluorescence measurement system. The kit includes a number of fluorescence standards i and their corrected and certified fluorescence spectra Ii(λ), whereby the fluorescence standards i are selected, so that their spectrally corrected fluorescence spectra Ii(λ) cover a broad spectral range with high intensity. The standards are characterized by large half-widths FWHMiof their bands of at least 1400 cm−1. According to the method of the invention, partial correction functions Fi(λ) are generated by forming the quotient of the measured fluorescence spectra Ji(λ) and the corresponding corrected fluorescence spectra Ii(λ), which are then combined to form a total correction function F(λ) for a broad spectral range. The combination factors αiare hereby computed by statistical averaging of consecutive partial correction functions Fi(λ) over only a predefined, limited overlap region λi/i+1±ΔλOLabout the mutual crossover wavelength λi/i+1.

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