Method and kit for ATP assays using cyclodextrin

Details Method and kit for ATP assays using cyclodextrin Method and kit for ATP assays using cyclodextrin

1994-11-23

1996-09-24

Horlick, Kenneth R.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

435 8, 435 39, 435259, 435810, 436 17, C12Q 100, C12Q 166

Patent

active

055589863

ABSTRACT:
A method of preparing an extract of an intracellular component, wherein cells are contacted with an extractant to generate an extract solution, which comprises contacting the solution with a cyclodextrin to neutralize the extractant. preferred extractants are cationic or other kinds of surfactants. The cyclodextrin is preferably used in solution. Preferred intracellular components are ATP which can, after neutralization of the extractant, be assayed by a firefly luciferase assay; and DNA or RNA which can, after neutralization of the surfactant, be amplified or further processed in other ways.

REFERENCES:
patent: 4956274 (1990-09-01), Khanna et al.
patent: 5004684 (1991-04-01), Simpson et al.
patent: 5032503 (1991-07-01), Khanna et al.
Virology, vol. 93, 1979, New York (N.Y., U.S.), pp. 260-264; P. F. Fignatti et al.: "Herpes Simplex virus DNA Isolation from Infected Cells with a Novel Procedure"; see abstract; see p. 260, right column, line 5-line 15.

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