Method and kit for analyzing target nucleic acid fragment

Details Method and kit for analyzing target nucleic acid fragment Method and kit for analyzing target nucleic acid fragment

2006-11-07

2006-11-07

Riley, Jezia (Department: 1637)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

C435S006120, C435S091200, C536S022100, C536S023100, C536S024300, C536S024330, C536S025320, C536S025600

Reexamination Certificate

active

07132266

ABSTRACT:
A method of analyzing a target nucleic acid fragment is provided that is capable of being performed simply and promptly, by anyone, using a compact device without requirement of specific technique or complex operation. The method comprises timely detection of a double-stranded nucleic acid fragment formed during the polymerase extension reaction due to a specific base sequence of the target nucleic acid fragment as increase of an electrochemical response under the existence of an electrochemically active intercalator; and the kit utilizes the method.

REFERENCES:
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patent: 6294670 (2001-09-01), Takenaka
patent: 6316230 (2001-11-01), Egholm et al.
patent: 6361951 (2002-03-01), Thorp et al.
patent: 2001/0021504 (2001-09-01), Makino et al.
patent: 2002/0068294 (2002-06-01), Takenaka
patent: 2003/0036072 (2003-02-01), Lee et al.
Takagi, M. (Pure Appl. Chem. (Oct. 2001), 73(10): 1573-1577).
Stratagene Catalog (1988), p. 39.
Takenaka et al. (Bull. Chem. Soc. Jpn. (1999) 72: 327-337).
Stratagene catalog 1988, p. 39.

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