Method and instrumentation for measuring fluorescence...

Optics: measuring and testing – By dispersed light spectroscopy – With sample excitation

Reexamination Certificate

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Reexamination Certificate

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06947134

ABSTRACT:
A Fluorescence Particle Spectrometer (FPS) performs real-time measurement of the fluorescence spectra of aerosol particles in the size range 1-10 μm diameter. The FPS has a sufficiently high sample rate (estimated to be a few liters/min) to measure aerosol within buildings (from 1 up to 600 particle fluorescence spectra per minute) at practical rates. A virtual impactor first concentrates aerosol particles, which are then drawn under negative pressure through an aerodynamic focusing nozzle in the inlet of the instrument, through the sample region, providing further concentration. The rate of particle spectra measured by the FPS increases significantly when the particle inlet is within a few meters of some common sources of indoor biological particles.

REFERENCES:
patent: 5030002 (1991-07-01), North, Jr.
patent: 5162863 (1992-11-01), Ito
patent: 6532067 (2003-03-01), Chang et al.
Ronald G. Pinnick, Steven C. Hill, Paul Nachman, and Gordon Videen, “Aerosol Fluorescence Spectrum Analyzer for Rapid Measurement of Single Micrometer-Sized Airborne Biological Particles”, Aerosol Science and Technology, 28:95-104 (1998).
Ronald G. Pinnick, Steven C. Hill, Paul Nachman, and J. David Pendleton, “Fluorescence Particle Counter for Detecting Airborne Bacteria and Other Biological Particles”, Aerosol Science and Technology, 23:653-664 (1995).
Paul Nachman, Gang Chen, R. G. Pinnick, Steven C. Hill, Richard K. Chang, Michael W. Mayo, and Gilbert L. Fernandez, “Conditional-Sampling Spectrograph Detection System for Fluorescence Measurements of Individual Airborne Particles”; Applied Optics, vol. 35, No. 7, Mar. 1, 1996.
Steven C. Hill, Ronald G, Pinnick, Paul Nachman Gang Chen, Richard K. Chang, Michael W. Mayo, and Gilbert L. Fernandez, “Aerosol-Fluorescence Spectrum Analyzer: Real-Time Measurement of Emission Spectra of Airborne Biological Particles”; Applied Optics, vol. 34, No. 10, Oct. 20, 1995.
Steven C. Hill, Ronald G. Pinnick, Stanley Niles, Nicholas F. Fell, Jr., Yong-Le Pan, Jerold Bottiger, Burt V. Bronk, Stephen Holler, and Richard K. Chang, “Fluorescence From Airborne Microparticles: Dependence on Size, Concentration of Fluorphores, and Illumination Intensity”, Applied Optics, vol. 40, No. 18, Jun. 20, 2001.
Steven C. Hill, Ronald G. Pinnick, Stanley Niles, Yong-Le Pan, Stephen Holler, Richard K. Chang, Jerold Bottiger, Bean T. Chen, Chung-Sing Orr, and Greg Feather, “Real-Time Measurement of Fluorescence Spectra From Single Airborne Biological Particles”, Field Analytical Chemistry and Technology, vol. 3, Issues 4-5, 1999.
Yong-Le Pan, Stephen Holler, and Richard K. Chang, “Single-Shot Fluorescence Spectra of Individual Micrometer_Sized Bioaerosols Illuminated By a 351- or 266-nm Ultraviolet Laser”, Optics Letters, vol. 24, No. 2, Jan. 15, 1999.

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