Method and formulation for lyophilizing cultured human cells to

Details Method and formulation for lyophilizing cultured human cells to Method and formulation for lyophilizing cultured human cells to

1997-06-27

2000-06-06

Prats, Francisco

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Method of storing cells in a viable state

4353723, 435372, C12N 502, C12N 500, C12N 508

Patent

active

060717459

ABSTRACT:
The present invention relates to methods for lyophilizing eukaryotic cells and isolating intact nucleic acids from such cells, and related kits for using the same.

REFERENCES:
patent: 4520113 (1985-05-01), Gallo et al.
patent: 5059518 (1991-10-01), Kortright
patent: 5409826 (1995-04-01), Maples et al.
patent: 5525715 (1996-06-01), Racaniello et al.
Chemical Abstracts 122 (13) :158688c.
Chomczynski, Piotr (1993) "A Reagent for the Single-Step Simultaneous Isolation of RNA, DNA and Proteins from Cell Tissue Samples", BioTechniques 15(3) :532-535.
Gill, Santokh S., et al. (1996) "Ensuring Revovery of Intact RNA from Rat Pancreas", Molecular Biotechnology, 6:359-362.
Jennings, Thomas, A. (1997) "Effect of formulation on lyophilizatoin, part 1", IVD Technology 3(1) :38-42.
Jennings, Thomas, A. (1997) "Effect of formulation on lyophilization, part 2", IVD Technology 3(2) :42-49.
Greaves, R.I.N. (1964) "Fundamental aspects of freeze-drying bacteria and living cells", Aspects Theoriques et Industriels de la lyophilisation, 407-410.

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