Method and formulation for creating kinase isoform separation

Details Method and formulation for creating kinase isoform separation Method and formulation for creating kinase isoform separation

1989-10-20

1992-12-15

Niebling, John

Chemistry: electrical and wave energy

Processes and products

Electrostatic field or electrical discharge

204299R, G01N 2726

Patent

active

051714108

ABSTRACT:
An improved gel formulation and method for high-voltage, short duration electrophoretic separation of isoforms of CK isoenzymes. The gel is an homogeneous mixture of agarose and a buffer having a molarity of about 0.05M. CK-MM and/or CK-MB isoforms are electrophoretically separated.

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Gorus et al., "A Sensitive Bioluminescent Immunoinhibition Test for CK-B Subunit Activity and a CK-MB Specific Elisa Compared; Correlation with Agarose Electrophoresis and Influence of CK-Isoenzyme Profile on Results," Clinical Chemistry, vol. 34, No. 7, pp. 1474-1478 (1988).
Puleo et al., "Sensitive Rapid Assay of Subforms of Creatine Kinase MB in Plasma" Clinical Chemistry, vol. 35, No. 7, pp. 1492-1495 (1989).

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