Method and erythrocyte preparation for reverse blood grouping

Details Method and erythrocyte preparation for reverse blood grouping Method and erythrocyte preparation for reverse blood grouping

1973-06-08

1976-05-11

Turner, V. D.

Drug, bio-affecting and body treating compositions

Radionuclide or intended radionuclide containing; adjuvant...

Molecular bilayer structure

23253TP, 424 3, 424 11, 424 12, 424 13, G01N 3100, G01N 3316

Patent

active

039564770

ABSTRACT:
Disclosed herein is a stable preparation of human erythrocytes for use in reverse blood typing comprising erythrocytes hemolyzed by gradual hypotonic hemolysis using a sequence of aqueous hypotonic buffers to form stroma wherein the stroma cells are in the form of discoid bodies having the reticular membrane of the cell substantially intact. The stroma reagent has been found to be highly stable and antigenic in the sense that it contains blood group antigens (carried on the stroma cells), and can be admixed with human blood serum or plasma to determine the presence of hemologous blood group antibodies.

REFERENCES:
patent: 2770572 (1956-11-01), Eldon
patent: 3666421 (1972-05-01), Price
patent: 3714345 (1973-01-01), Hirata
Williams, et al., Methods in Immuno. & Immunochem., Acd. Press N.Y., Vol. I 1967, pp. 339-346.
Dodge, Arch. of Biochem. & Biophy. Vol. 100, 1963 p. 119.
Tullis, Blood Cells & Plasma Proteins, Acdemic Press, N.Y., 1953 pp. 52-53.
Benyo, Chem. Abs., Vol. 75, Aug. 30, 1971 p. 264 Ab. No. 61480b.
Benyo, Hungary, Abstract No. T/1731, Mar. 1971 1 p.
Poulik, Nature, Vol. 208, Nov. 27, 1965 p. 874.
Dodge, Arch. Biochem. & Biophys. Vol. 100, 1963 pp. 119-130.

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